![]() You might get some different colors due to variation in pH, but other than that, you're ok. The Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer. The ladder will be separated fine, and it will transfer fine - so if all you're using it for is to tell when your gel has run far enough and whether transfer has occurred sufficiently, any pre-stained ladder will be fine. This only comes into play if you're using the standards to find the molecular weight of your protein. However, as I said, the migration of the pre-stained standards is sensitive to pH, so you'd need to calibrate your pre-stained standard with a non-stained standard to get the apparent molecular weights as they behave in your system. Choose prestained, tagged, or fluorescent standards. The PureView Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western. There's no reason why any ladder would not work with this gel system. Select from an extensive range of protein ladders for SDS PAGE, western blotting, IEF and 2D-SDS PAGE. Fluorescent western blotting is growing in popularity because it allows the ability to perform multiplex detection, where multiple proteins can be detected at the same time. This gel uses chloride as the mobile anion other gel chemistries use other anions. It usually has a stacking gel of pH 6.8 and a separating gel of pH 8.8. The Western blot X-ray film (if using chemiluminescence) can be overlaid onto the blotted membrane and the positions of the protein ladder bands marked by pen on the X-ray film. A Tris-HCL gel is just the original Laemmli gel, around since the 1970's.
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